论文部分内容阅读
真核翻译起始因子IF4E(initiation factor 4E)可通过与m RNA的5’帽子端结合,在蛋白质的翻译起始过程中扮演重要角色。草菇Volvariella volvacea是一种富有商业价值的食用真菌,其生长发育与蛋白质的合成代谢密切相关。本文通过鉴定草菇编码IF4E的基因(Vv-IF4E),并根据生物信息学和实时荧光定量数据分析Vv-IF4E及相关转录因子基因表达量的变化规律。结果显示,草菇Vv-IF4E基因上游存在较多顺式作用元件,基因存在4种可变剪切体,只有一种具有翻译起始因子的保守结构域。预测开放阅读框(ORF)长度为3 083bp,所编码蛋白分子量为87.1k Da,存在35个磷酸化位点。Vv-IF4E蛋白结构有异于拟南芥IF4E,但与皱木耳Auricularia delicata IF4E相似。实时荧光定量结果表明,Vv-IF4E与转录因子YRR1、ECM22存在极强的共表达规律。
The eukaryotic translation initiation factor IF4E (initiation factor 4E) plays an important role in the translation initiation of proteins by binding to the 5 ’cap of m RNA. Volvariella volvacea is a fungus with commercial value. Its growth and development are closely related to the protein synthesis. In this paper, the gene Vv-IF4E and Vf-IF4E were identified and analyzed by bioinformatics and real-time fluorescence quantitative analysis. The results showed that there are more cis-acting elements in the upstream of Vv-IF4E gene of V. rufipogon. There are four kinds of variable splicing genes in the upstream of Vv-IF4E gene and only one kind of conserved domain with translation initiation factor. The predicted open reading frame (ORF) length was 3 083 bp, the molecular weight of the encoded protein was 87.1 kDa, and 35 phosphorylation sites existed. The Vv-IF4E protein structure is different from the Arabidopsis IF4E but similar to the Auricularia delicata IF4E. Real-time fluorescence quantitative results showed that Vv-IF4E and transcription factors YRR1, ECM22 there is a strong rule of co-expression.