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目的:建立血竭素的含量测定方法。方法:HPLC 法,色谱柱为 DiamonsilTM C_(18)(250 mm×4.6 mm,5μm);流动相为乙腈-0.05 mol·L~(-1)磷酸二氢钠溶液(35:65);检测波长为440nm;柱温35℃;流量1 ml·min~(-1)。结果:血竭素含量在2.96～14.80μg·ml~(-1)范围内具有良好线性关系(r=0.999 8),平均回收率为98.54%,RSD=1.36%。结论:本法简便、快速、准确,可用于大七厘散的质量控制。
Objective: To establish a method for determining the content of urhodin. Method: HPLC method, DiamonsilTM C_(18) (250 mm×4.6 mm, 5 μm) as the chromatographic column; Acetonitrile-0.05 mol·L -1 sodium dihydrogen phosphate solution (35:65) as the mobile phase; Detection wavelength 440nm; column temperature 35°C; flow rate 1 ml·min~(-1). RESULTS: There was a good linearity in the range of 2.26～14.80μg·ml -1 for the reference of plasma Dracin content (r=0.999 8). The average recovery was 98.54% and RSD was 1.36%. Conclusion: This method is simple, rapid and accurate and can be used for quality control of Daqiqi San.