Ames试验是目前检测基因突变常用检测系统之一,所用的菌株为组氨酸突变型鼠伤寒沙门氏菌,此菌如保存不当,则特性易发生变异。因此,解决本菌保存中存在的一些问题十分必要。目前在国内主要的保存方法有冷冻干燥保存法和低温冷冻保存法。50年代初stamp研究了一种明胶片保存细菌方法,此法在国外已被中小型实验室采用,就我国目前现状,冷冻干燥保存虽是长期保存细菌最理想方法,但需特定的设备条件,成批大量冷冻,成本高,一般实验室做不到;低温冷冻保存,需-80℃低温冰箱 Ames test is one of the commonly used detection systems for detecting gene mutations. The strain used is histidine-mutated Salmonella typhimurium. If the strain is improperly preserved, the characteristics are easily mutated. Therefore, to solve some problems existing in the preservation of this bacterium is very necessary. At present, the main methods of preservation in the country are freeze-drying method and cryopreservation method. In the early 1950s, a stamping method was studied for gelatine preservation bacteria. This method has been adopted by small and medium laboratories in foreign countries. For the current status of our country, freeze-dried preservation is the most ideal method for long-term preservation of bacteria, but requires specific equipment conditions, Large quantities of frozen, high cost, the general laboratory can not do; cryogenic preservation, to -80 ℃ low temperature refrigerator