目的对ＤＮＡ酸解后甲基绿—派洛宁染色法进行改良和应用。方法收集我室常规病理活检标本，已明确病理诊断的消化道恶性肿瘤：胃癌、食管癌，应用改良后的染色法，进行组织切片染色。结果染出切片色彩鲜艳，组织结构清晰，能显示多种组织细胞成份，尤其能同时显示肿瘤细胞的增殖活力，分化程度和肥大细胞的形态及成熟程度。结论改良后的染色法对比度强，染色时间短，操作步骤简便易掌握，适用于组织形态学研究和临床病理外检的辅助诊断。 Objective To improve and apply the methyl green-pyronin staining method after DNA acid hydrolysis. Methods The routine pathological biopsy specimens from our department were collected. Pathologically diagnosed malignant tumors of the digestive tract, including gastric cancer and esophageal cancer, were treated with modified staining and stained with tissue sections. Results The stained slices were vivid in color, clear in structure, and capable of displaying a variety of tissue cell components. In particular, they were able to show the proliferation activity of tumor cells, the degree of differentiation, and the morphology and maturity of mast cells. Conclusion The improved staining method has high contrast ratio, short dyeing time, simple and easy-to-handle procedures, and is suitable for the auxiliary diagnosis of histomorphology and clinical pathology.