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~(60)Coγ-线照射人外周血转化淋巴细胞后,即刻经碱性蔗糖梯度(5～20%〕超离心沉降分析,结果表明随照射剂量(1～6Krad)增大,DNA单链断裂亦增加。3Krad照射细胞经与自体血清37℃保温在2小时以内,DNA断裂链重接分子随保温时间的延长而逐渐增加,然未见完全修复。若继续保温至8小时后,DNA重接分子又发生断裂,至24小时后已重接好的DNA沉降峰几乎消失,并在离心管口出现DNA降解分子,同时细胞活力降低,较保温前下降28%。可以设想DNA重接分子的再断裂是一种不可逆的生化变化,且与细胞死亡有关。
After the ~(60)Co gamma-ray irradiation of human peripheral blood lymphocytes, it was immediately subjected to ultracentrifuge sedimentation with an alkaline sucrose gradient (5-20%). The results showed that single-stranded DNA breaks with increasing irradiation dose (1 to 6 Krad). In addition, the 3Krad irradiated cells were incubated with autologous serum at 37°C for less than 2 hours, DNA strand repetitive molecules gradually increased with the incubation time, however, no complete repair was observed, and if the incubation was continued for 8 hours, the DNA was reconnected. The molecules broke again, and after 24 hours, the re-connected DNA sedimentation peaks almost disappeared and DNA degrading molecules appeared at the orifice of the centrifuge tube, and the cell viability was reduced by 28% compared to the pre-incubation. Fracture is an irreversible biochemical change and is associated with cell death.