论文部分内容阅读
目的分析乙型肝炎患者血清中抗核抗体(ANA)的检测结果并分析其与性别、年龄、肝功能和HBV DNA浓度的相关性。方法收集182例乙型肝炎患者、80例健康体检者的血清标本,采用间接免疫荧光法(IIF)检测抗核抗体(ANA),使用OLYMPUS全自动生化分析仪检测肝功能,实时荧光定量PCR法检测HBV DNA。结果 ANA在乙型肝炎组的阳性率(22.5%)显著高于正常对照组(3.8%),差异有统计学意义(P<0.01)。ANA-IIF在女性乙型肝炎患者的阳性率(34.5%)显著高于男性(11.6%),差异有统计学意义(P<0.01)。ANA-IIF阳性在45岁以上乙型肝炎患者中较常见。ANA-IIF阳性组的ALB滴度显著低于ANA-IIF阴性组,差异有统计学意义(P<0.01),ANA-IIF阳性组的ALT、AST和TBIL浓度高于ANA-IIF阴性组,差异均有统计学意义(P<0.05)。ANA-IIF阳性组的HBV DNA浓度显著高于ANA-IIF阴性组,差异有统计学意义(P<0.01)。结论部分乙型肝炎患者体内存在ANA,且性别、年龄、肝功能及HBV DNA可能会影响ANA的出现。
Objective To analyze the results of anti-nuclear antibody (ANA) in serum of patients with hepatitis B and analyze its correlation with gender, age, liver function and HBV DNA concentration. Methods Serum samples of 182 patients with hepatitis B and 80 healthy controls were collected. Anti-nuclear antibody (ANA) was detected by indirect immunofluorescence (IIF), liver function was detected by OLYMPUS automatic biochemical analyzer, real-time fluorescence quantitative PCR Detection of HBV DNA. Results The positive rate of ANA in hepatitis B group (22.5%) was significantly higher than that in normal control group (3.8%) (P <0.01). The positive rate of ANA-IIF in female patients with hepatitis B (34.5%) was significantly higher than that of male (11.6%), the difference was statistically significant (P <0.01). ANA-IIF positive in the 45-year-old hepatitis B patients are more common. The ALB titers in ANA-IIF positive group were significantly lower than those in ANA-IIF negative group (P <0.01), and the concentrations of ALT, AST and TBIL in ANA-IIF positive group were higher than those in ANA-IIF negative group All were statistically significant (P <0.05). The HBV DNA concentration in ANA-IIF positive group was significantly higher than that in ANA-IIF negative group (P <0.01). Conclusion Some patients with hepatitis B have ANA in their body, and gender, age, liver function and HBV DNA may affect the appearance of ANA.