为研究甘蓝CMS不育系材料PM的败育机理,以甘蓝不育系PM和保持系PF为试材,用透射电镜观察其小孢子发育的整个过程。结果表明:不育系在花粉母细胞时期形状不规则,严重影响了减数分裂的正常进行;在单核小孢子期,绒毡层细胞不解体且异常膨大,挤压小孢子使其变形,粘连在一起,并出现了严重的质壁分离现象,最后降解的小孢子和绒毡层细胞充斥着整个花粉囊,导致花药败育。 In order to study the mechanism of PM abortion in CMS CMS in cabbage, the male sterile line PM and maintainer line PF were used as materials to observe the microspore development by transmission electron microscopy. The results showed that the male sterile lines were irregular in pollen mother cells and seriously affected meiosis. In the microspore stage, the tapetal cells did not disintegrate and expanded abnormally, They are conglutinated together and present severe plasmolysis. The resulting degraded microspores and tapetum cells are flooded with the entire pollen sac, which leads to anther abortion.