Square wave voltammetric quantification of folic acid,uric acid and ascorbic acid in biological matr

来源 :Journal of Pharmaceutical Analysis | 被引量 : 0次 | 上传用户:cznay
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Nowadays, modified electrodes with metal nanoparticles have appeared as an alternative for the electroanalysis of various compounds. In this study, gold nanoparticles(GNPs) were chosen as interesting metal nanoparticles for modifying carbon paste electrode(CPE). GNPs and the gold nanoparticles-modified carbon paste electrode(GNPs/CPE) were characterized by UV–Vis spectroscopy, transmission electron microscopy(TEM) and scanning electron microscopy(SEM). GNPs/CPE as a simple and sensitive electrode was used to study three important biological molecules: folic acid(FA), uric acid(UA) and ascorbic acid(AA). Square wave voltammetry(SWV) was used as an accurate technique for quantitative measurements. A good linear relation was observed between anodic peak current(ipa) and FA(5.2 × 10~(-6)– 2.5 × 10~(-5)M), UA(1.2 × 10~(-6)– 2.1 × 10~(-5)M) and AA(1.2 × 10~(-6)– 2.5 × 10~(-5)M) concentrations in simultaneous determination of these molecules. Nowadays, modified electrodes with metal nanoparticles have been as an alternative for the electroanalysis of various compounds. In this study, gold nanoparticles (GNPs) were chosen as interesting metal nanoparticles for modifying carbon paste electrode (CPE). GNPs and the gold nanoparticles-modified Carbon paste electrodes (GNPs / CPE) were characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). GNPs / CPE as a simple and sensitive electrode was used to study three important biological molecules: folic acid (FA), uric acid (UA) and ascorbic acid (AA). Square wave voltammetry (SWV) was used as an accurate technique for quantitative measurements. A good linear relation was observed between anodic peak current (ipa) and FA 10 -6 -6.5 × 10 -5 M), UA (1.2 × 10 -6 -6 2.1 × 10 -5 M) and AA (1.2 × 10 -6) - 2.5 × 10 ~ (-5) M) concentrations in simultaneous determination of these molecules.
其他文献
酰氯化合物、Fe Cl2(0.05倍摩尔量)在THF中,加入苄基氯化锌(1.25倍摩尔量),25℃反应0.5~4 h,制得相应的酰化产物酮,10例收率50%~88%;如为苯基氯化锌,50℃反应2~4 h,相应的酰
5月7日,按照《乘用车企业平均燃料消耗量核算办法》(工信部2013年第15号公告),工信部将各汽车企业递交的“2013年企业平均燃料消耗量执行情况年度报告”进行了公示,引起业内震动。  公示问题多  事实上,“2013年度乘用车企业平均燃料消耗量情况表”(以下简称“公示情况”)并非汽车行业的靓丽成绩单,甚至在某种程度上,更象是工信部在“自揭其短”。  据相关数据显示,不论国产车企还是进口车企,20
采用射频热等离子体制备了Ni含量为50 mol%的Ni-CeO_2催化剂,考察了其在甲烷部分氧化反应中的催化活性.在催化剂制备过程中,采用板功率为52 kVA的射频等离子体火炬将Ni(直径
本文在没有铵离子存在下,于乙酸缓冲溶液中,对Yb(Ⅲ)-NO_3,Yb(Ⅲ)-NO_2体系的脉冲极谱性能进行了研究,并与经典极谱实验此较,确认由于Yb(Ⅲ)作为多价阳离子能促使NO_3~-和NO_
甲基砜或甲磺酰胺用双(二亚苄基丙酮)钯和2-二环己基膦-2,4,6-三异丙基联苯为催化剂,2,2,6,6-四甲基哌啶·氯化锌·氯化锂为碱,和芳基溴经加热或微波处理得到苄基砜或苄磺酰
铜试剂分离-偶氮胂Ⅲ光度法测定稀土总量,具有干扰少、准确可靠的优点,但其稳定性欠佳,需要逐个显色测定是其不足。其稳定性差的主要原因在于以氟化物退色的空白不稳定及铬
由上海市高等教育局、江西省机械工业厅联合主持的“稀土元素分组测定的新试剂:新方法推广应用交流会”于1981年12月4日至9日在上海召开。这是一次专业性比较强的会议,有78
目前,有机化合物中硫的测定广泛采用的还是氧瓶燃烧,以钍啉[苯-2-胂酸钠-(1-偶氮-1)-二-羟基萘-3.6-二磺酸钠]为指示剂,高氯酸钡滴定硫酸根的方法,然而磷、氟及许多金属元素
在弱酸性溶液中,用饱和溴水将HCN氧化为BrCN。BrCN同吡啶作用使吡啶环裂开,转化为戊烯二醛。戊烯二醛同巴比妥酸作用,生成红紫色聚亚甲基染料. 一主要试剂分析纯冰乙酸;饱和
一前言美国Raytheon公司在1956年生产了工业规模的热解石墨制品,成功地应用于宇航工业。我国于六十年代初,开始研制热解石墨材料。我们在国内外工作的基础上,进行了热解石墨