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供试验用的细胞是用牛白血病病毒(BLV)慢性感染的胎羊肾细胞(FLK)和在玻璃器皿内用BLV转化的绵羊成纤维细胞(SF-28)。大多数FLK细胞表面附着糖蛋白51(GF51)抗原。试验所用4种疫苗为:经BLV感染的FLK细胞培养液中提取的GP51及P24,用戊二醛固定的FLK细胞和SF-28细胞。经BLV感染的FLK细胞培养液用0.01%N-乙酰乙烯亚胺灭活,37℃作用24小时(经合胞体试验证明已无感染性BLV),再用50%硫酸铵沉淀后,浓缩100倍,将沉淀物
Cells for testing were fetal sheep kidney cells (FLK) chronically infected with bovine leukemia virus (BLV) and sheep fibroblasts (SF-28) transformed with BLV in glassware. Most FLK cell surface glycoprotein 51 (GF51) antigen. The four vaccines used in the experiment were GP51 and P24 extracted from BLV-infected FLK cell culture medium, FLK cells fixed with glutaraldehyde and SF-28 cells. The BLV-infected FLK cell culture medium was inactivated with 0.01% N-acetylethyleneimine and incubated at 37 ° C for 24 hours (syphilis test proved non-infectious BLV), then precipitated with 50% ammonium sulfate and then concentrated 100 times , The precipitate