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目的采用免疫组化标记人血白蛋白微泡造影剂,观察其在兔肝脏VX2肿瘤造影实质相中的病理分布,目的是解释肝肿瘤超声造影显影的病理机制。方法10只荷VX2肝肿瘤新西兰白兔,经耳缘静脉团注人血白蛋白氟碳微泡造影剂,剂量0.04ml/kg;造影剂推注前设定为0时间点,静脉团注后分别在30s、3min、5min、10min时间点快速取肝脏组织及肿瘤组织各2~3块。用鼠抗人HAS-11抗体对标本进行免疫组化染色,SP法分析造影剂在肝和VX2肿瘤组织中的分布。结果观测时间点内,检测样本中VX2肿瘤组织偶见散在的造影剂免疫阳性反应复合物表达,而造影剂免疫阳性复合物在肝组织内分布的顺序和表达程度不同:注射后30s,仅肝细胞有散在的阳性表达,注射后3~10min,阳性反应产物在肝细胞中逐渐增多、增强,而在肝窦内的阳性棕黄色反应颗粒在窦壁表达逐渐增多;团注后造影剂的免疫反应在汇管区则以3min、5min时表达最为明显,10min时几乎无阳性表达。结论人血白蛋白微泡在肝细胞、肝窦内、窦壁以及汇管区周围均有不同程度的分布,其在肝内分布随着时间延长有从窦内向窦壁、肝细胞转移的倾向。
Objective To observe the pathological distribution of human albumin microbubbles labeled with immunohistochemistry in rabbit VX2 tumor in vivo and to explain the pathological mechanism of contrast-enhanced liver tumor imaging. Methods Ten New Zealand white rabbits with VX2 liver tumor were injected with albumin fluorocarbon microvesicle contrast agent through the ear vein at the dose of 0.04ml / kg. The contrast medium was injected into the vein before the bolus injection. The liver tissues and tumor tissues were rapidly taken from 2 to 3 at 30s, 3min, 5min and 10min respectively. Immunohistochemical staining was performed on mouse anti-human HAS-11 antibody, and SP method was used to analyze the distribution of contrast medium in liver and VX2 tumor tissues. Results During the observation time point, the expression of immunosorbent positive reaction complexes scattered in the VX2 tumor tissue was detected occasionally in the test samples, and the order and degree of expression of the immunogen positive immunocomplexes in the liver tissue were different: 30 s after injection, only the liver Cells were scattered in the positive expression of 3 ~ 10min after injection, the positive reaction products gradually increased in liver cells, increased, and in the sinusoidal positive brown yellow particles in the sinusoidal expression gradually increased; group injection of contrast agent after the immunization The reaction in the portal area at 3min, 5min most obvious expression, almost no positive expression at 10min. Conclusions Human albumin microbubbles are distributed in varying degrees in hepatocytes, sinusoids, sinus wall and portal area, and their distribution in the liver tends to shift from sinus to sinus wall and hepatocytes over time.