采用化学、色谱、凝胶电泳等方法,对经酚水法提取、苯酚-氯仿-石油醚法进一步纯化的4株口腔厌氧菌内毒素脂多糖进行了化学组分分析,结果发现4种内毒素物理和外观性状良好,纯度高,4种内毒素主要由糖类、脂肪酸类和磷组成;糖类由氨基糖、酸性糖KDO和中性糖组成;脂肪酸由饱和、不饱和、分枝和羟基脂肪酸组成。SDS—PAGE分析表明,4种内毒素均为半粗糙型,具有内源异质性。 The chemical constituents of 4 lipopolysaccharide from oral anaerobic bacteria were purified by phenol-water extraction and phenol-chloroform-petroleum ether method using chemical, chromatographic and gel electrophoresis methods. The results showed that within 4 The physical and appearance properties of the toxin are good and the purity is high. The four endotoxins are mainly composed of sugars, fatty acids and phosphorus. The sugars are composed of amino sugars, acidic sugars KDO and neutral sugars. The fatty acids consist of saturated, unsaturated, branched and Hydroxy fatty acid composition. SDS-PAGE analysis showed that all four endotoxins were semi-rough and had endogenous heterogeneity.