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文章用自制的明胶肽灌胃饲养SD大鼠实施营养干预,通过电刺激方法诱发动物疲劳,在评价明胶肽抗疲劳功效的基础上,检测疲劳动物前肢腓肠肌肌肉中的ATP含量、ATP酶活性和肌电积分。实验结果显示:和蒸馏水饲养组对比,明胶肽能延长动物运动时间,降低疲劳后的血乳酸和尿素氮含量,提高肝肌糖原含量,具有显著的抗疲劳功效;ATP酶活性实验组为3.92,对照组为2.33,ATP含量实验组为31.71,对照组为11.58;肌电积分实验组明显比对照组多(2.39>1.32);分析以上数据,明胶肽抗肌肉疲劳的可能通路是在增加糖原含量的基础上,作为信使改善ATP酶活性,提高ATP生成量,增强了肌肉的做功能力,达到抗疲劳功效。
In this paper, SD rats were intragastrically fed with homemade gelatin peptide for nutritional intervention, and electrical fatigue was induced by electrical stimulation. On the basis of evaluating the anti-fatigue effect of gelatin peptides, ATP content and ATPase activity in gastrocnemius muscle of forelimbs EMG integral. The experimental results showed that compared with distilled water feeding group, gelatin peptide could prolong the exercise time, reduce the content of blood lactic acid and urea nitrogen and improve the content of hepatic muscle glycogen after fatigue, and had significant anti-fatigue effect. The activity of ATPase in experimental group was 3.92 , The control group was 2.33, the ATP content was 31.71 in the experimental group and 11.58 in the control group; the EMG score was significantly higher in the experimental group than in the control group (2.39> 1.32). Based on the above data, the possible pathway of gelatin peptide against muscle fatigue was to increase the sugar Based on the original content, as a messenger to improve ATPase activity, increase ATP production, enhance muscle work ability, to anti-fatigue effect.