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【目的】解剖分析烟青虫Helicoverpa assulta成虫脑的结构,并构建脑三维结构数字化模型。【方法】利用神经突触蛋白抗体,对烟青虫成虫脑进行免疫组织化学染色标记,利用共聚焦激光扫描显微镜获得脑扫描数码图像,并结合三维图像分析软件对烟青虫脑结构进行识别分析,构建三维模型。【结果】突触蛋白抗体免疫染色将烟青虫脑和颚神经节的神经髓区域清晰标记出来。烟青虫成虫脑与颚神经节愈合而成为一体,中间具有一个孔洞,为食道穿过的通道。脑主要包括前脑、中脑和后脑3部分。依据染色标记结果识别和构建了至少16个脑神经髓结构。这些神经髓包括边界清晰的视叶、前视结节、蕈形体、中央复合体和触角叶及其亚结构。除此之外,还包括围绕这些神经髓的其他前脑神经髓区域,但这部分前脑神经髓内部边界模糊,不容易细分,而将其与颚神经节区域作为一个整体标记为中间脑,占脑总神经髓的55.05%。【结论】识别出烟青虫脑的主要功能结构区域,并成功构建了三维模型。该研究结果为进一步研究烟青虫脑接收、处理和整合感觉信息及调控行为的机制奠定了解剖学基础,并为研究烟青虫或其他昆虫脑结构发育、变异和重塑提供结构形态和体积大小依据。
【Objective】 Anatomical analysis of the brains of Helicoverpa assulta adult tobacco and the construction of digital model of brain three-dimensional structure. 【Method】 The synaptophysin antibody was used to detect the brain of tobacco budworm, which was labeled by immunohistochemistry. The digital image of brain was obtained by confocal laser scanning microscope. The brain structure of tobacco budworm was identified by three-dimensional image analysis software, Three-dimensional model. 【Results】 The immunostaining of synaptophysin antibody clearly marked the neuronal regions of the brain and palatal ganglion of tobacco budworm. Tobacco wormworm adult brain and jaw ganglion healing and become integrated in the middle with a hole for the esophagus through the channel. The brain mainly includes the forebrain, midbrain and brain 3 parts. Based on the results of the staining, at least 16 neuropsychiatric structures were identified and constructed. These neurons include clear-cut optic lobes, foreskin nodules, mushroom bodies, central complexes and antennae and their sub-structures. In addition to this, there are other forebrain neuromedial regions surrounding these neurons, but the inner boundaries of this part of the forebrain neuron are blurred and not easily subdivided and are labeled as the middle brain with the region of the jaw ganglia as a whole , Accounting for 55.05% of the total brain neurons. 【Conclusion】 The main functional structure regions of tobacco budworm were identified and a three-dimensional model was successfully constructed. The results provide an anatomical basis for further study on the mechanism of tobacco sensitivities receiving, handling and integrating sensory information and regulating behavior in tobacco and provide structural basis and volume for the study of brain structure development, mutation and remodeling of tobacco budworm or other insects .