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目的探讨血管瘤患儿血清血管内皮生长因子(VEGF)、雌二醇(E2)及尿碱性成纤维细胞生长因子(bF-GF)水平的变化,以鉴别血管瘤和血管畸形,并为血管瘤临床分期提供依据。方法选择血管瘤及血管畸形患儿138例,其中增生期血管瘤(增生期血管瘤组)60例,消退期血管瘤(消退期血管瘤组)38例,血管畸形(血管畸形组)40例。采用ELISA法检测各组血清VEGF及尿bFGF水平,采用电化学发光法、使用自动免疫分析仪检测各组血清E2水平,并与正常对照组进行对照。结果增生期血管瘤组患儿血清VEGF、E2及尿bFGF水平显著高于消退期血管瘤组、血管畸形组、正常对照组(均P<0.01)。消退期血管瘤组、血管畸形组患儿血清VEGF水平与正常对照组比较差异均无统计学意义(均P>0.05)。消退期血管瘤组患儿血清E2水平显著高于血管畸形组、正常对照组(P<0.05)。消退期血管瘤组、血管畸形组患儿尿bFGF水平与正常对照组比较差异均无统计学意义(均P>0.05)。增生期血管瘤组女性血清VEGF、E2及尿bFGF水平均显著高于男性,但差异均无统计学意义(均P>0.05)。结论采用ELISA法检测血清VEGF、尿液bFGF及应用电化学发光法检测血清E2简便、易行、可靠,可为临床鉴别血管瘤和血管畸形,并为临床划分血管瘤分期提供依据。
Objective To investigate the changes of serum vascular endothelial growth factor (VEGF), estradiol (E2) and urinary basic fibroblast growth factor (bF-GF) in children with hemangiomas to identify hemangiomas and vascular malformations, Tumor provides the basis for clinical staging. Methods Thirty-eight children with hemangiomas and vascular malformations were enrolled, including 60 cases of proliferative hemangioma (proliferative hemangioma group), 38 cases of regression hemangioma (retrograde hemangioma group), 40 cases of vascular malformation (vascular malformation group) . Serum levels of VEGF and urinary bFGF in each group were detected by ELISA. The level of serum E2 was detected by the method of electrochemiluminescence and the level of serum E2 in each group was detected by ELISA. Results The levels of VEGF, E2 and urinary bFGF in hyperplastic hemangioma group were significantly higher than those in hemangiomas, vascular malformation group and normal control group (all P <0.01). There was no significant difference in the serum VEGF levels between the hemangiomas and the vascular malformations in the regression group (all P> 0.05). The level of serum E2 in patients with regression hemangioma was significantly higher than that in vascular malformation group and normal control group (P <0.05). There was no significant difference in urinary bFGF level between the patients with hemangiomas and vascular malformations in the regression group and the control group (all P> 0.05). The levels of serum VEGF, E2 and urinary bFGF in proliferative hemangioma group were significantly higher than those in male, but the difference was not statistically significant (all P> 0.05). Conclusions The detection of serum VEGF and urine bFGF by ELISA and the detection of serum E2 by electrochemiluminescence are simple, easy and reliable, which can be used to differentiate the hemangiomas and vascular malformations clinically and provide the basis for clinical staging of hemangiomas.