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目的研究丹黄通脉方(DHTMD)对大鼠颈动脉粥样硬化性狭窄模型炎性反应的影响。方法将大鼠随机分为四组,每组10只。三组采用高脂加高蛋氨酸复合饲料喂养4 W后行颈总动脉球囊拉伤术法制备大鼠颈动脉狭窄模型,空白组给予普通饲料喂养,其余三组给予高脂加高蛋氨酸复合饲料喂养,阳性药组给予辛伐他汀2.5 mg/kg,DHTMD组给予DHTMD 20 g/kg生药量,配制成10 mL/kg的药液,灌胃给药,一天两次。继续喂养8 W后,检测血脂四项、TNF-α、CRP、ICAM-1及Hcy的水平。结果与空白组比较,4W时其它三组大鼠的TC和Hcy明显升高(P<0.05);治疗8 W后与模型组比较,DHTMD组和阳性药组的Hcy、CRP、TNF-α、ICAM-1、TC、TG、LDL-C水平均有显著降低(P<0.05);与阳性药组比较,DHTMD组的Hcy、CRP、TNF-α、ICAM-1及LDL-C水平降低且有统计学意义(P<0.05)。结论 DHTMD具有调节血脂代谢和抗炎作用,能够抑制颈动脉粥样硬化的形成,防治缺血性脑血管疾病。
Objective To study the effect of Danhuang Tongmai Fang (DHTMD) on the inflammatory response of carotid atherosclerotic stenosis model in rats. Methods The rats were randomly divided into four groups of 10 rats. Three groups were fed with high-fat and high-methionine diet for 4 W and then carotid artery stenosis model was established by carotid artery balloon injury. The blank group was fed with normal feed, while the other three groups were given high-fat and high-methionine diet The positive drug group was given simvastatin 2.5 mg / kg, DHTMD group was given DHTMD 20 g / kg crude drug dose, formulated as 10 mL / kg of liquid, intragastric administration, twice a day. After 8 weeks of continuous feeding, the levels of four lipids, TNF-α, CRP, ICAM-1 and Hcy were detected. Results Compared with the blank group, TC and Hcy in the other three groups were significantly increased at 4W (P <0.05). Compared with the model group, the levels of Hcy, CRP, TNF-α, The levels of ICAM-1, TC, TG and LDL-C in the DHTMD group were significantly lower than those in the positive drug group (P <0.05). The levels of Hcy, CRP, TNF-α, ICAM-1 and LDL- Statistical significance (P <0.05). Conclusion DHTMD can regulate blood lipid metabolism and anti-inflammatory effects, can inhibit the formation of carotid atherosclerosis, prevent ischemic cerebrovascular disease.