【摘 要】
：
实验应用脉冲场电泳方法获得鸡核DNA,根据鸡线粒体DNA序列设计引物,以总DNA和核DNA为模板通过PCR扩增、测序和筛选鸡BAC文库方法.结果表明:鸡核DNA中普遍存在线粒体基因16S R
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实验应用脉冲场电泳方法获得鸡核DNA,根据鸡线粒体DNA序列设计引物,以总DNA和核DNA为模板通过PCR扩增、测序和筛选鸡BAC文库方法.结果表明:鸡核DNA中普遍存在线粒体基因16S RNA、tRNA-Leu和部分ND1的同源序列;在鸡的总DNA为模板PCR中,扩增产物含有核线粒体DNA( numtDNA)产物;同时发现,该段numtDNA序列存在2个碱基突变位点.研究结果提示,在涉及PCR相关技术的mtDNA研究中,numtDNA的影响是不能忽视的.
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