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目的寻找发现红树林底泥放线菌(N2010-37)中抗肿瘤活性成分。方法放线菌(N2010-37)发酵菌丝体经95%乙醇提取,溶剂分级萃取,应用多种柱色谱分离和谱学分析方法对醋酸乙酯萃取部位化学成分进行分离鉴定。结果从醋酸乙酯部位分离得到1个新的单环内酯和2个蒽酮类化合物,依次鉴定为(3S,4R,7R,8R,9S)-3,8-二羟基-4,7,9-三甲基-2,6-环壬二酯(1)、1-甲氧基-2-羟基-3-甲基蒽醌(2)、1,6,8-三羟基-3-甲基蒽醌(3)。结论化合物1为新化合物,体外抗肿瘤实验表明,化合物1和3对人类慢性髓性白血病细胞系K562细胞株具有一定的细胞毒活性。
Objective To find out the antitumor activity of actinomycete (N2010-37) in mangrove sediment. Methods The mycelium of Actinomycetes (N2010-37) was extracted with 95% ethanol and fractionated by solvent extraction. The chemical constituents in the extraction of ethyl acetate were separated and identified by a variety of column chromatography and spectroscopic methods. Results One new monocyclic lactone and two anthrone compounds were isolated from the ethyl acetate fraction and identified as (3S, 4R, 7R, 8R, 9S) -3,8-dihydroxy- Trimethyl-2,6-cyclonononyl ester (1), 1-methoxy-2-hydroxy-3-methylanthraquinone (2), 1,6,8-trihydroxy- Anthraquinone (3). Conclusion Compound 1 is a new compound. In vitro antitumor experiments show that compounds 1 and 3 have certain cytotoxic activity on human chronic myeloid leukemia K562 cell line.