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目的:研究甲氧苄啶的电化学性质,初步探讨甲氧苄啶在电极上的响应机理,并用电化学方法对其进行检测。方法:在0.1mol.L-1KNO3底液中,用玻碳电极循环伏安法和示差脉冲扫描伏安法研究甲氧苄啶的电化学性质,并用示差脉冲扫描伏安法测定甲氧苄啶的含量。结果:甲氧苄啶的氧化峰电位是1.3V,氧化峰电流与甲氧苄啶的浓度在2.0×10-5～4.2×10-3mol.L-1范围内呈良好的线性关系。该测定方法的检出下限(3S/N)为4.0×10-6mol.L-1。用标准加入法测定回收率范围在96.1%～101.6%,RSD为3.2%(n=5)。结论:甲氧苄啶在1.3V处的氧化为4电子参加的受扩散控制的不可逆过程。甲氧苄啶的电化学测定方法能很好地用于实际样品的测定而且结果与2005年版中国药典方法一致。
OBJECTIVE: To study the electrochemical properties of trimethoprim and to explore the response mechanism of trimethoprim on the electrode. The electrochemical mechanism of trimethoprim was also investigated electrochemically. Methods: The electrochemical properties of trimethoprim were studied by cyclic voltammetry and differential scanning calorimetry in 0.1mol.L-1KNO3 solution. The trimethoprim Content. Results: The oxidation peak potential of trimethoprim was 1.3V. The oxidation peak current was linear with the concentration of trimethoprim in the range of 2.0 × 10-5 ~ 4.2 × 10-3mol.L-1. The detection limit (3S / N) of this measurement method was 4.0 × 10 -6 mol.L-1. The recoveries ranged from 96.1% to 101.6% with RSD of 3.2% (n = 5) by standard addition method. Conclusion: The oxidation of trimethoprim at 1.3 V is an irreversible diffusion controlled process involving 4 electrons. Trimethoprim electrochemical determination method can be well used in the determination of the actual sample and the results with the 2005 edition of the Chinese Pharmacopoeia method.