目的:探讨小飞蓬药材定性、定量检测方法。方法:采用TLC鉴别小飞蓬中的槲皮素,HPLC测定小飞蓬中槲皮素的含量。结果:TLC以正己烷-乙酸丁酯-甲酸(6∶5∶1)为展开系统,可以清晰地检测槲皮素;HPLC采用日本岛津ShimadzuC18(4.6 mm×250 mm,5μm)色谱柱,流动相-甲醇-0.4%磷酸(47∶53),检测波长257 nm,流速1 mL.min-1,柱温30℃,槲皮素在0.504～1.134μg呈良好线性关系,平均回收率分别为97.97%%,RSD分别为1.2%。结论:该法可作为小飞蓬药材的定性、定量检测方法。 Objective: To investigate the method of qualitative and quantitative detection of Xiao Fei Peng medicinal materials. Methods: Quercetin was identified by TLC and the content of quercetin in Xiao-Fei Peng was determined by HPLC. Results: QuEChERS could be detected clearly by TLC with n-hexane-butyl acetate-formic acid (6: 5: 1) as developing system. HPLC was performed on a Shimadzu C18 (4.6 mm × 250 mm, 5 μm) The detection wavelength was 257 nm, the flow rate was 1 mL.min-1, the column temperature was 30 ℃ and the quercetin was in the range of 0.504-1.134 μg. The average recoveries were 97.97 %%, RSD were 1.2%. Conclusion: This method can be used as a qualitative and quantitative detection method of Radix phyllanthus.