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用细胞化学的方法，对经过不同时间低温诱导小麦细胞表面糖蛋白的动态变化进行了标记定位电镜观察。小麦幼苗经２－３℃下低温锻炼后，细胞表面的糖蛋白层出现明显的增厚，随之开始逐渐地减少变薄，有些细胞表面的糖蛋白层部分脱落于细胞间隙。随低温诱导处理时间不同，细胞表面糖蛋白层表现出的由少量到增厚，最后部分的脱落减少变薄这一有规律的变化现象表明，低温诱导能够引起小麦细胞表面糖蛋白的合成积累，抗寒性较强的冬小麦较非抗寒性的春小麦，糖蛋白层增厚的现象更加明显，耐受低温条件的时间也较长。
Cytochemical methods were used to observe the dynamic changes of the surface glycoproteins of wheat cells induced by hypothermia at different time points. After the wheat seedlings were subjected to low temperature exercise at 2-3 ° C, the glycoprotein layer on the cell surface was obviously thickened, and then began to gradually reduce and thinning. Some of the cell surface glycoprotein layer partially detached from the cell gap. With the different time of low temperature induction treatment, the regular change of the cell surface glycoprotein from small volume to thicken, and the final part of the shedding reduced and thinned showed that low temperature induction can cause the accumulation of surface glycoprotein synthesis, The winter wheat with stronger cold-resistance was more obvious than the non-cold-tolerant spring wheat with thickening of glycoprotein, and the longer the temperature was.