检测白血病或肿瘤细胞多药耐药基因(mdr 1)有多种方法,其中以RT-PCR法相对更灵敏、简单和特异.目前国内多用β_2微球蛋白(β_2MG)为内参照,以mdrl/β_2MG比值进行相对定量.现已发现β_2MG可受肿瘤影响.本文以细胞高表达的组织蛋白H 3.3(H 3.3)为内参照,建立了RT-PCR mdrl基因表达方法,并对临床10例标本进行检测.本文探讨了内参照应用的价值、存在的问题以及临床应用的意义. There are multiple methods for detecting multidrug resistance gene (mdr 1) in leukemia or tumor cells, of which the RT-PCR method is relatively more sensitive, simple and specific. Currently, many domestic β 2 microglobulin (β 2 MG) is used as an internal reference and mdrl/ Relative quantification of β 2 MG ratio has been found. It has been found that β 2 MG is affected by tumors. In this paper, the high expression of tissue protein H 3.3 (H 3.3) as an internal reference, established a RT-PCR mdrl gene expression method, and 10 clinical specimens Detection. This article explores the value, existing problems, and implications of clinical applications for internal reference applications.