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目的建立毛细管电泳-电化学发光法分离并检测复方磷酸可待因中三种成分,磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱含量的新方法。方法基于碱性介质中,复方磷酸可待因溶液中三种主要成分对联吡啶钌在铂电极上的电化学发光信号的增敏作用,且增敏强度和药物浓度成线性关系,与毛细管电泳联用,建立了毛细管电泳-电化学发光法分离并检测其含量的新方法。结果在优化的实验条件下,确定三种药物对照品的线性范围、线性方程和检出限。通过对浓度为1.0×10-5kg/L的磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱对照品进行12次平行测定,其ECL发光强度的RSD值分别为2.89%,3.76%,3.32%。结论该方法可用于复方磷酸可待因口服溶液中三种主要成分的含量测定,待测样品中的基质不干扰测定,方法的回收率分别为100.4%,101.9%,99.8%(n=5)。
OBJECTIVE To establish a new method for the separation and determination of three components of codeine phosphate, codeine phosphate, bromfenamin maleate and ephedrine hydrochloride by capillary electrophoresis and electrochemiluminescence. Methods Based on the sensitization effect of three main components of codeine phosphate compound in alkaline medium on electrochemiluminescent signal of platinum bipyridyl diphosphate, the sensitization intensity and drug concentration were linearly correlated with capillary electrophoresis A new method for the separation and detection of its content by capillary electrophoresis-electrochemiluminescence was established. Results Under the optimized experimental conditions, the linear range, linear equation and detection limit of the three drugs were determined. The RSD values of ECL luminescence intensity were 2.89% and 3.76%, respectively, by parallel determination of codeine phosphate, bromobenzene maleate and ephedrine hydrochloride reference substance for 12 times at a concentration of 1.0 × 10-5kg / L, 3.32%. Conclusion The method can be used to determine the content of three major components in the codeine phosphate compound solution. The matrix in the test sample does not interfere with the determination. The recoveries of the method are 100.4%, 101.9% and 99.8%, respectively (n = 5) .