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通过限制性培养条件和连续继代培养,筛选获得了一组高效稳定分解小麦秸秆的复合菌群FWD1。该菌群在10 d内对小麦秸秆的分解率达到76.92%,发酵液中主要成分为乙酸、丙酸、丁酸和乙醇。利用第二代高通量测序技术对复合菌群和菌种来源土壤样品的细菌组成进行分析,结果表明FWD1中主要含有变形菌门、厚壁菌门和拟杆菌门,在种分类水平上,Clostridium sp.BNL1100,uncultured Alcaligenes sp.,uncultured Alcaligenaceae bacterium和Brevundimonas diminuta为优势菌株。通过连续继代培养富集了变形菌门和厚壁菌门,更细化分类水平上富集了以Clostridium sp.BNL1100为主的纤维素降解菌。菌群通过菌种之间的协同作用,共同维持了体系的稳定。研究结果为明确菌群降解机理和提高木质纤维素降解效率提供了基础。
Through restrictive culture conditions and continuous subculture, a group of complex flora FWD1 with efficient and stable decomposition of wheat straw was obtained. The bacterial population decomposition rate of wheat straw reached 76.92% within 10 days. The main components of fermentation broth were acetic acid, propionic acid, butyric acid and ethanol. The second generation of high-throughput sequencing technology was used to analyze the bacterial composition of the composite bacteria and soil samples from the strain. The results showed that FWD1 mainly contained Proteobacteria, Firmicutes and Bacteroidetes, Clostridium sp.BNL1100, uncultured Alcaligenes sp., Uncultured Alcaligenaceae bacterium and Brevundimonas diminuta are dominant strains. Proliferation bacteria and Firmicutes were enriched in successive subcultures, and cellulose-degrading bacteria mainly containing Clostridium sp.BNL1100 were enriched at a more refined classification level. Through the synergy between the strains, the flora maintained the stability of the system. The results provide a basis for clarifying the mechanism of microbial degradation and improving the degradation efficiency of lignocellulose.