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免疫酶斑点法(Dot--ELISA)是近年在ELISA基础上建立起来的微量蛋白检测技术,它除具有ELISA的简便、敏感、特异等特点外,尚有样品用量极少,节约抗原的特点。我们将此法用于筛选抗间日疟原虫(P.v.)单抗,克服了抗原来源限制给检测带来的困难,使检测效率大大提高。
Dot - ELISA is a trace protein detection technique established on the basis of ELISA in recent years. In addition to the simple, sensitive and specific characteristics of ELISA, there are few samples used to save the antigen. We used this method to screen anti-P. malaria parasite (P.v.) monoclonal antibody, which overcomes the difficulties of detection caused by the antigen source restriction and greatly improves the detection efficiency.