The aim of the study was to evaluate the long-term outcomes of pelvic floor muscle (PFM) rehabilitation in males with lifelong premature ejaculation (PE),using intravaginal ejaculatory latency time (IELT) and the self-report Premature Ejaculation Diagnostic Tool (PEDT) as primary outcomes.A total of 154 participants were retrospectively reviewed in this study,with 122 completing the training protocol.At baseline,all participants had an IELT ＜60 s and PEDT score ＞11.Participants completed a 12-week program of PFM rehabilitation,including physio-kinesiotherapy treatment,electrostimulation,and biofeedback,with three sessions per week,with 20 min for each component completed at each session.The effectiveness of intervention was evaluated by comparing the change in the geometric mean of IELT and PEDT values,from baseline,at 3,6,and 12 months during the intervention,and at 24 and 36 months postintervention,using a paired sample 2-tailed t-test,including the associated 95％ confidence intervals.Of the 122 participants who completed PFM rehabilitation,111 gained control of their ejaculation reflex,with a mean IELT of 161.6 s and PEDT score of 2.3 at the 12-week endpoint of the intervention,representing an increase from baseline of 40.4 s and 17.0 scores,respectively,for IELT and PEDT (P ＜ 0.0001).Of the 95 participants who completed the 36-month follow-up,64％ and 56％ maintained satisfactory ejaculation control at 24 and 36 months postintervention,respectively.