以红莲型 (HL )细胞质雄性不育系和保持系为材料 ,构建了水稻线粒体基因组的 BAC文库。每个文库保存约2 30 0个菌落 ,外源插入片段介于 9～ 2 5 kb之间。以线粒体基因为探针对文库进行菌落原位杂交验证 ,均筛选到了阳性克隆。构建的两个文库为进一步研究水稻线粒体基因组的结构特点 ,为克隆与红莲型水稻细胞质雄性不育相关的线粒体基因奠定了基础 The BAC library of rice mitochondrial genome was constructed with HL (HL) cytoplasmic male sterile line and maintainer line. Approximately 2 30 0 colonies were preserved in each library, with exogenous inserts ranging between 9 and 25 kb. The mitochondrial gene was used as a probe to verify the colony in situ hybridization. All the positive clones were screened. Two libraries were constructed to further study the structural characteristics of rice mitochondrial genome and lay the foundation for the cloning of mitochondrial genes related to cytoplasmic male sterility