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为探索娄彻氏链霉菌(D74)和密旋链霉菌(Act12)及其混合菌剂对玉米生长的影响,采用皿内发芽试验、沙培试验及小区试验观察供试链霉菌无细胞发酵滤液及活菌制剂种子包衣对玉米生物学性状、叶片诱导酶活性、光合作用、穗性状、产量及品质的影响.结果表明:供试链霉菌无细胞发酵滤液处理玉米种子可促进玉米胚根、胚轴及幼苗生长,提高玉米幼苗叶片诱导酶活性.D74发酵液稀释1000倍处理可使玉米胚根长度、胚轴长度及须根数较对照分别增加43.4%、26.4%和100.7%(P<0.05);D74原液可使玉米叶片多酚氧化酶(PPO)活性较对照增加40.2%.Act12发酵液稀释100倍处理可使玉米胚根长度、胚轴长度和须根数较对照分别增加36.3%、36.3%和117.5%(P<0.05),玉米幼苗总鲜质量、根系鲜质量分别较对照增加31.1%、36.6%(P<0.05);Act12稀释10及1000倍处理玉米种子可使玉米叶片PPO活性分别较对照增加38.1%和39.5%(P<0.05).混合菌剂种子包衣具有以下作用:1)促进玉米根系发育;2)显著增强叶片光合能力;3)显著改善穗性状并提高籽粒产量;4)显著促进籽粒灌浆速度;5)明显提高灌浆期玉米叶片诱导酶活性.表明供试链霉菌制剂包衣玉米种子可显著影响玉米的生物学特性、光合生理及生化代谢,刺激根系发育,促进玉米生长,提高产量.
In order to explore the effects of Streptomyces ultimus (D74) and Act12 and their mixed agents on the growth of maize, germination test, sand culture test and cell plot test were used to observe the cell-free fermentation filtrate And the effects of seed coating of viable preparations on maize biological traits, leaf-inducing enzyme activities, photosynthesis, ear traits, yield and quality were studied.The results showed that the treatment of maize seeds with Streptomyces atrophase cell-free fermentation filtrate could promote maize radicle, Hypocotyls and seedlings and increased the activity of induced enzymes in leaves of maize seedlings.Dilution of D74 fermentation broth by 1000 times could increase the radicle length, hypocotyl length and number of fibrous roots in maize by 43.4%, 26.4% and 100.7%, respectively (P <0.05 ); D74 liquid could increase the PPO activity by 40.2% compared with the control.Act12 fermentation broth diluted 100 times could increase the radicle length, hypocotyl length and number of fibrous roots by 36.3%, 36.3% % And 117.5% (P <0.05). The total fresh weight and fresh weight of maize seedling increased by 31.1% and 36.6% respectively compared with the control (P <0.05) An increase of 38.1 over the control % And 39.5%, respectively (P <0.05). Seed coating with mixed fungi had the following effects: 1) promoting root development of maize; 2) significantly enhancing photosynthetic capacity of leaves; 3) significantly improving ear traits and increasing grain yield; 5) significantly increased maize leaf induction enzyme activity during grain filling stage, indicating that the test of Streptomyces test dressing can significantly affect maize biological characteristics, photosynthetic physiology and biochemical metabolism, stimulate root development and promote maize growth and increase Yield.