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定性定量分析双孢磨菇不同形态发生阶段不同细胞组织的可溶性蛋白。在所研究的三个商业品种中,其中两个品种菌盖的可溶性蛋白含量比其他细胞组织高是有意义的。通过用聚丙酰胺凝胶电泳研究菌盖、菌柄,菌环或初生和次生菌丝体的蛋白质剖面亦不相同。在菌环中细胞色素氧化酶和酪氨酸酶的活性最高,在菌盖的细胞组织中最低。细胞色素氧化酶和过氧化酶异构酶的带状范型在不同形态发生期不同。在菌盖、菌环和菌柄提取物中细胞色素氧化酶的酶原相同。可是,在菌丝体提取物中缺乏细胞色素氧化酶和酪氨酸酶这两个带。测定菌丝体提取物过氧化酶的凝胶染色却显出最大量的区带。从菌丝体到原基及最终产生菌伞的形态发生中,这些酶的作用已被讨论。尽管双孢蘑菇的商品重要(Sange)embach(=A·brumescens peck),但是从菌丝体生化反应的结果导致形成一个成熟的担子果是仅仅知道的部分。在这一性状研究中,主要欠缺的是结成担子果所要求的环境和营养条件很严格。在实验室无菌条件下初始的担子果或果体的形成是非常困难的。相反,在某些担子菌中如Schizophyll-um commune Fr·和Coprinus的一些种,在容器里的形态发生能被利用一定的物理化学因素控制(Madelin,1956a,b;Raper and Krongelb 1958)。在菇房,蘑菇生长在消过毒,供给其生长发育木质素营养丰富的堆肥。木质素被酚氧化酶分解,随后酚的产物被利用,这在初始启蒙期间比在菌丝生长期间多(Gerrits 1968)。而且营养范型与原基外表的转变同时发生。为了研究导致担子菌从一个水平生长菌丝到形成一个垂直或竖直担子果因素的性质。我们已在蘑菇的不同发育阶段,研究它的可溶性蛋白和苯酚一氧化酶。过氧化酶和细胞色素氧化酶异构酶带状范型已通过聚丙烯酰胶电泳研究。
Qualitative and quantitative analysis of the different morphological stages of Agaricus bisporus soluble protein in different cell tissues. Of the three commercial cultivars studied, it is of interest that the soluble protein content of the caps of two of the two cultivars is higher than that of other cell tissues. The protein profile of the cap, stipe, loop, or primary and secondary mycelia is also different by studying polyacrylamide gel electrophoresis. In the bacterial loop, cytochrome oxidase and tyrosinase activity of the highest, the lowest in the bacterial cap tissue. The band pattern of cytochrome oxidase and peroxidase isomerase differs at different morphological stages. In the cap, loop and stipe extract cytochrome oxidase of the same zymogen. However, the mycelium extract lacks two bands of cytochrome oxidase and tyrosinase. Gel staining of the mycelium extract peroxidase showed the largest amount of zone. The role of these enzymes has been discussed in the morphogenesis of mycelium to primordium and eventually bacterial parasite. Although Sanger embach (= A. brumescens peck) is the commodity of Agaricus bisporus, the result of the biochemical reaction from the mycelium results in the mere formation of a mature burden. In the study of this trait, what is mainly lacking is that the environmental and nutritional conditions required for the formation of a burden are very strict. The initial burden of fruit or fruit body formation under laboratory conditions is very difficult. In contrast, in some basidiomycetes, some species of Schizophyllum commune Fr. And Coprinus, the morphogenesis in the vessel can be controlled by certain physicochemical factors (Madelin, 1956a, b; Raper and Krongelb 1958). In the mushroom house, mushrooms grow in disinfectant and supply their nutrient-rich compost with growth and development of lignin. Lignin is broken down by phenol oxidase and then phenol products are utilized, which is greater during initial enlightenment than during mycelial growth (Gerrits 1968). And nutrition paradigm and the transformation of the original base appearance at the same time. In order to study the nature of the factors responsible for the growth of basidiomycetes from one level to the formation of a vertical or vertical basidiomycete. We have studied its soluble protein and phenol-oxidase at different stages of the development of mushrooms. Peroxidase and cytochrome oxidase isozyme bands have been studied by polyacrylamide gel electrophoresis.