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随着荧光素、各种酶类、放射性同位素等被用作免疫组化技术中的标记物,胶体金的应用也引起了学者们的注意。胶体金作为细胞化学的标记物这一技术是从本世纪七十年代发展起来的。1971年Faulk和Taylor首先将兔抗沙门氏菌抗血清与胶体金颗粒结合,用直接免疫细胞化学技术检测沙门氏菌的表面抗原。1974、1975年Romano报道了间接法检测技术。1974年Bauer等报道凝集素—金复合物应用。1975、1977年Horisberger等将胶体金引入扫描电镜,作为细胞化学标记物。1978年Geoghegan
With the use of fluorescein, various enzymes, radioisotopes and the like as markers in immunohistochemistry, the use of colloidal gold has drawn the attention of scholars. Colloidal gold as a marker of cytochemistry this technology has developed from the seventies of this century. In 1971, Faulk and Taylor first combined rabbit anti-Salmonella antiserum with colloidal gold particles to detect Salmonella surface antigens using direct immunocytochemistry. In 1974, 1975, Romano reported the indirect method. In 1974, Bauer et al. Reported the use of lectin-gold complexes. In 1975, 1977, Horisberger et al. Introduced colloidal gold into scanning electron microscopy as a cytochemical marker. 1978 Geoghegan