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应用微生物技术可以对乙草胺污染土壤进行修复,经前期分离筛选及鉴定,已获得高效乙草胺降解菌株Bacillus subtilus L3,为进一步明确其对乙草胺的实际降解效果及对污染土壤的修复作用,对L3开展盆栽效果研究。试验考察了不同降解菌浓度和施用时间对乙草胺降解率的影响,以及降解菌的施用对土壤中可培养的常见微生物数量、酶活、营养元素及敏感作物小麦生长的影响等。结果表明,终浓度为5×108CFU/g土的菌液对乙草胺的降解率最高,50 d后对乙草胺的降解率为92.65%;施用L3菌液后,污染土壤微生物的数量有较大程度的恢复;蔗糖酶、脲酶、蛋白酶、过氧化氢酶活力分别提高100%、260%、56%与6%;土壤的全氮、速效磷、有效钾、有机质分别提高77%、159%、698%和274%;小麦地上部分较对照组增高117%,总鲜重增重56%,根长增长27%。该结果证实菌株L3具有良好的乙草胺降解效果和土壤修复能力,为该菌株在土壤污染治理及修复中的实际应用奠定了理论基础。
Microbial technology can be used to repair acetochlor-contaminated soil. After pre-screening and identification, Bacillus subtilus L3 has been obtained. In order to further clarify the actual degradation of acetochlor and the remediation of contaminated soil Effect on the L3 to carry out potted effect. The effects of different degrading bacteria concentration and application time on the degradation rate of acetochlor and the effects of degrading bacteria on the amount of common microorganisms, enzyme activities, nutrients and the growth of sensitive crops wheat in soils were investigated. The results showed that the degradation rate of acetochlor was the highest at the concentration of 5 × 108CFU / g soil, and the degradation rate of acetochlor was 92.65% after 50 days. The amount of microorganisms in the soil after applying L3 strain was The activity of sucrase, urease, protease and catalase increased by 100%, 260%, 56% and 6% respectively. The contents of total nitrogen, available phosphorus, available potassium and organic matter increased by 77% %, 698% and 274% respectively. The above-ground part of wheat increased by 117%, the total fresh weight gain by 56% and the root length by 27%. This result confirms that strain L3 has good acetochlor degradation and soil remediation ability, which lays a theoretical foundation for the practical application of this strain in soil pollution control and remediation.