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利用透射显微镜(TEM)观察亚洲玉米螟Ostrinia furnacalis(Guene)幼虫取食了表达Cry1Ab杀虫蛋白的转Bt基因玉米心叶组织后中肠的组织病理变化,以探讨转Bt基因玉米对亚洲玉米螟的致病机理,为其合理、安全和持续利用提供理论依据。结果表明:亚洲玉米螟取食Bt玉米后中肠细胞及其细胞器发生了明显的病变。取食Bt玉米12h后中肠细胞开始病变,首先微绒毛脱落、内质网开始肿胀,24h后内质网肿胀、增多,杯状细胞杯腔增大,48h后微绒毛大量脱落,细胞开始空泡化,随着取食时间的增加,细胞空泡化程度加剧,在感染前期细胞间的病变程度差异较大。微绒毛脱落、内质网肿胀断裂是在多数取食Bt玉米的亚洲玉米螟中肠细胞发生的普遍病变。由此表明,人工修饰的Cry1Ab基因导入到玉米染色体组中所表达的杀虫蛋白可使玉米螟幼虫中肠细胞发生病变,最终导致其死亡。
Transmission electron microscopy (TEM) was used to observe the histopathological changes of the midgut of the transgenic Bt corn (Ostrinia furnacalis (Guenéee) larvae fed with Cry1Ab insecticidal protein) The pathogenic mechanism of corn borer provides a theoretical basis for its reasonable, safe and sustainable use. The results showed that there were obvious lesions of midgut cells and organelles of Asian corn borer after feeding on Bt corn. The midgut cells started to lesion 12h after ingestion of Bt corn. First, the microvilli shed and the endoplasmic reticulum began to swell. After 24h, the endoplasmic reticulum swelled and increased, the goblet cells enlarged, and the microvilli shed off after 48h. The cells began to empty With the increase of feeding time, the degree of cell vacuolization increased, and the degree of lesion between cells in the early stage of infection was quite different. Microvilli shedding, endoplasmic reticulum swelling and rupture are common lesions of intestinal cells in the Asian corn borer that consumes most Bt corn. Thus, the introduction of the artificial modified CrylAb gene into the maize genome express the insecticidal protein in the corn borer larval midgut cells disease, eventually leading to its death.