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目的 探讨外周血 型前胶原肽 ( PIIIP)、 型胶原 ( CIV)、层粘蛋白 ( LN)、透明质酸 ( HA)、丙二酰二醛 ( MDA)、过氧化物歧化酶 ( SOD)在肝损伤、肝纤维化形成过程中的作用。方法 以四氯化碳( CCL4)诱导大鼠形成肝纤维化模型 ( 1 2周 ) ,并用秋水仙碱灌胃以缓解肝纤维化进程 ,采用放免法和化学反应法分别检测 PIIIP、CIV、LN、HA和 SOD、MDA,同时检测肝功能 ( TB、TP、ALB、ALT) ,并对肝组织进行免疫组化检查。结果 血清 PIIIP、CIV、LN、HA含量随着肝纤维化的严重程度而显著增加 ,模型组 >缓解组 >对照组 ( P <0 .0 5 ) ;SOD和 MDA在肝纤维化中的表达呈负相关 ( Y=2 4 3.90 - 2 .2 96X,γ=-0 .85 94) ,SOD活性以模型组最低 ,缓解组次之 ,均低于对照组 ,而 MDA则相反 ( P <0 .0 5 )。结论 ( 1 ) PII-IP和 CIV、HA、LN在肝纤维化进程中发挥重要作用 ,是肝纤维化程度的诊断和疗效观察的重要指标 ;( 2 )MDA、SOD可能参与肝损伤和修复过程 ,可反映肝功损害程度和肝纤维化的活跃程度
Objective To investigate the expression of PIIIP, CIV, LN, HA, MDA and SOD in liver Injury, the role of liver fibrosis in the formation process. Methods Liver fibrosis was induced by carbon tetrachloride (CCL4) in rats (12 weeks), and colchicine was used to relieve the progression of liver fibrosis. Radioimmunoassay and chemical reaction were used to detect the expression of PIIIP, CIV, LN , HA and SOD, MDA, liver function (TB, TP, ALB, ALT) were detected at the same time. Immunohistochemistry was performed on the liver tissue. Results Serum levels of PIIIP, CIV, LN and HA increased significantly with the severity of liver fibrosis. The model group> remission group> control group (P <0.05); the expression of SOD and MDA in liver fibrosis was Negative correlation (Y = 24.390-2.22 96X, γ = -0.854 94). SOD activity was the lowest in model group and second in remission group, which was lower than that in control group (P <0. 0 5). Conclusions (1) PII-IP, CIV, HA and LN play an important role in the process of hepatic fibrosis, which is an important index for the diagnosis and therapeutic effect of liver fibrosis. (2) MDA and SOD may participate in the process of liver injury and repair , Can reflect the degree of liver damage and the degree of liver fibrosis