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以克山病病区粮配成基础饲料，另在基础饲料中分别补充Ｓｅ或ＶＥ，或Ｓｅ＋ＶＥ喂养大鼠，在细胞及亚细胞水平上以Ｃａ代谢为主研究并比较了Ｓｅ和ＶＥ在克山病病因中的作用。测量了心肌细胞和心肌线粒体的Ｃａ代谢及有关指标、心肌线粒体能量转换功能及心肌组织自由基含量。结果表明，在低Ｓｅ病区粮中补充Ｓｅ或ＶＥ均能在一定程度上预防病区粮中致病因素对心肌细胞及线粒体的损伤；并且补充Ｓｅ或ＶＥ均能使心肌组织中自由基含量减少。提示Ｓｅ和ＶＥ是通过清除体内过量自由基预防细胞和线粒体的损伤的。但值得注意的是，实验中所用病区粮ＶＥ含量不低于甚至高于非病区对照粮，在低Ｓｅ情况下，所补ＶＥ的量需要相当大（如本实验中补充２００μｇ／ｇ）才能较明显地预防心肌细胞和心肌线粒体的损伤。通过对这些结果的分析，进一步肯定低Ｓｅ是克山病形成的重要因素之一。
Keshan disease ward allocated to the basic diet, and the other in the basal diet were supplemented with Se or VE, or Se + VE fed rats at the cellular and subcellular levels to Ca metabolism mainly Se and VE in grams The role of mountain etiology. Ca metabolism of myocardial cells and myocardial mitochondria and related indicators, myocardial mitochondrial energy conversion and free radical content in myocardium were measured. The results showed that supplementation of Se or VE in the low Se disease grain could prevent the damage of cardiomyocytes and mitochondria caused by pathogenic factors in the grain of disease in the ward to a certain extent. Supplementation of Se or VE could make the contents of free radicals cut back. It is suggested that Se and VE prevent cell and mitochondrial damage by eliminating excess free radicals in the body. However, it is noteworthy that the VE content of the ward food used in the experiment is not lower than even higher than the non-ward control grain. In the case of low Se, the amount of VE to be supplemented needs to be quite large (for example, 200 μg / g in this experiment) In order to more obvious prevention of myocardial cells and myocardial mitochondrial damage. Through the analysis of these results, it is further confirmed that low Se is one of the important factors in the formation of Keshan’s disease.