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目的:比较合成辣椒素(N-Vanillylnonanamide)诱导白血病细胞K562及其多药耐药K562/ADM、肝癌HepG2细胞凋亡的作用。方法:以K562细胞、K562/ADM细胞和HepG2细胞为靶细胞,采用噻唑蓝(MTT)比色法检测细胞增殖活性,流式细胞法测定细胞周期和AnnexinⅤ/PI双染色检测细胞凋亡。结果:MTT示合成辣椒素可抑制白血病细胞和HepG2细胞的增殖,根据同时期半数抑制浓度(IC50)示HepG2细胞最敏感,K562细胞较K562/ADM细胞敏感。合成辣椒素可阻滞细胞周期。合成辣椒素诱导24小时后AnnexinⅤ/PI双染色显示白血病细胞和肝癌细胞凋亡率升高;白血病敏感细胞株和耐药细胞株的对合成辣椒素敏感性相似。结论:合成辣椒素可抑制白血病和肝癌细胞的增殖并诱导其凋亡;辣椒素对药物敏感和耐受的白血病细胞的作用相似。
OBJECTIVE: To compare the effect of synthetic vanilloid (N-Vanillylnonanamide) on the apoptosis of leukemia cell K562, its multidrug-resistant K562/ADM, and hepatoma HepG2 cells. Methods: K562 cells, K562/ADM cells and HepG2 cells were used as target cells. MTT colorimetric assay was used to detect the cell proliferation activity. Flow cytometry was used to determine cell cycle and Annexin V/PI double staining was used to detect cell apoptosis. RESULTS: MTT assay showed that capsaicin inhibited the proliferation of leukemia cells and HepG2 cells. According to the half-time inhibitory concentration (IC50), HepG2 cells were most sensitive and K562 cells were more sensitive than K562/ADM cells. Synthetic capsaicin blocks the cell cycle. The double staining of AnnexinV/PI after 24 hours of induction with synthetic capsaicin showed that the apoptosis rate of leukemia cells and hepatoma cells increased; the sensitivity of leukemia sensitive cell lines and drug resistant cell lines to synthetic capsaicin was similar. CONCLUSION: Capsaicin can inhibit the proliferation of leukemia and hepatocellular carcinoma cells and induce apoptosis. Capsaicin has similar effects on drug-resistant and tolerant leukemia cells.