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目的:评估马甲子乙酸乙酯提取物(Ethyl acetate extract of Paliurus ramosissimus,EAEPR)对溃疡性结肠炎(Ulcerative Colitis,UC)的影响以及抗炎作用。方法:灌胃给予马甲子乙酸乙酯提取物0.8、1.6、3.2 g/kg,采用二甲苯致小鼠耳肿胀模型和小鼠棉球肉芽肿模型,评价其抗炎作用。以3%硫酸葡聚糖钠(Dextran sulfate sodium salt,DSS)溶液自由饮用7d复制实验性小鼠UC模型,自饮用DSS溶液当天开始灌胃给予马甲子乙酸乙酯提取物,剂量同上,连续7d,观察小鼠的一般状态,并进行结肠炎疾病指数(DAI)评分;第8d眼眶采血后脱颈椎处死动物,测定结肠长度并进行组织病理学观察,ELISA检测血清肿瘤坏死因子α(TNF-α)水平。结果:马甲子乙酸乙酯提取物各剂量均可显著抑制二甲苯所致小鼠耳肿胀和棉球肉芽肿形成;1.6、3.2 g/kg组能改善实验性UC小鼠一般状况,抑制体重降低和DAI评分增高,并能显著改善肠道水肿和肠道组织损伤,减轻炎细胞浸润,显著降低血清TNF-α水平。结论:马甲子乙酸乙酯提取物具有抗炎作用,可减轻DSS所致实验性小鼠UC病变严重程度,其作用可能与下调TNF-α等炎性因子分泌有关,是一种有效的UC治疗药物。
Objective: To evaluate the effect of Ethyl acetate extract of Paliurus ramosissimus (EAEPR) on ulcerative colitis (UC) and anti-inflammatory effects. Methods: Echinacea purpurea ethyl acetate extract was orally administered at 0.8, 1.6 and 3.2 g / kg respectively. The anti-inflammatory effects of xylene-induced mouse ear swelling model and mouse cotton ball granuloma model were evaluated. The model of experimental UC mice was freely drank with 3% Dextran sulfate sodium salt (DSS) solution for 7 days, and the ethyl acetate extract was administered orally from the day when the DSS solution was drank. The dosage was the same as above for 7 days , And the colitis disease index (DAI) score was observed. On the 8th day after the orbital blood sampling, the animal was sacrificed by cervical dislocation, the length of the colon was determined and histopathological observation was performed. The levels of tumor necrosis factor-α (TNF- α) )Level. Results: Ethyl acetate extract at different doses significantly inhibited xylene-induced mouse ear swelling and granuloma formation. 1.6 and 3.2 g / kg groups improved the general condition of mice with UC and inhibited body weight loss And DAI score increased, and can significantly improve intestinal edema and intestinal tissue damage, reduce inflammatory cell infiltration, significantly lower serum TNF-α levels. Conclusion: Ethyl acetate extract has the anti-inflammatory effect and can reduce the severity of UC lesions in experimental mice induced by DSS. The effect may be related to the down-regulation of the secretion of inflammatory factors such as TNF-α and is an effective UC treatment drug.