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应用Goldsmith报道的方法稍加改进,建立了从人及兔大网膜中抽提脂质血管生长因子（LAF）的流程,发现不管是人或兔的LAF均可使兔角膜形成新生毛细血管网,而皮下脂肪抽提物及PBS均无此作用。将大网膜和皮下脂肪抽提物同时作高效薄层层析（HPTLC）,发现大网膜抽提物中有一特异条带,不同于皮下脂肪抽提物,紫外光谱分析表明其吸收峰在250 nm。还研究了LAF对小鼠胸腺细胞增殖及对PHA诱导IL-2产生的影响,结果表明具有抑制作用且呈剂量依赖关系。
Applying the method reported by Goldsmith with slight improvement, a procedure of extracting lipid vascular endothelial growth factor (LAF) from human and rabbit omentum was established. It was found that either human or rabbit LAF could make rabbit cornea form new capillary network , While subcutaneous fat extract and PBS did not have this effect. The omentum and subcutaneous fat extract at the same time for high-performance thin layer chromatography (HPTLC), found in the omentum extract has a specific band, different from the subcutaneous fat extract, UV spectroscopy showed that the absorption peak in the 250 nm. The effect of LAF on the proliferation of mouse thymocytes and the production of IL-2 induced by PHA was also studied. The results showed that LAF had an inhibitory effect in a dose-dependent manner.