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为探讨活性氧对人类精子DNA的损伤作用 ,分别用 0、0 .0 6 2 5、0 .12 5、0 .2 5、0 .5及 1.0mM的H2 O2 和0、0 .1、1.0、2 .0、5 .0mM的 β NADPH在体外条件下处理精子 ,用单细胞凝胶电泳试验检测外源性H2 O2 和内源性O·-2 引起的精子细胞DNA链断裂。结果显示 :用 1.0mMH2 O2 或 5 .0mMNADPH处理精子 30min至 4h ,彗星细胞百分率显著增加 ,平均彗尾也显著增长 ,并有明显的作用时间 效应关系。精子细胞用 0 .0 6 2 5mM～ 1.0mMH2 O2 或 0 .1～ 5 .0mMNADPH处理 1h ,彗星细胞百分率与对照组比较显著增加 ,平均彗尾显著增长 ,并有明显的剂量 效应关系。这些研究结果表明 :外源性H2 O2 和β NADPH在体外条件下刺激精了细胞产生的内源性O·-2 可引起精子DNA链断裂
In order to explore the effect of reactive oxygen species (ROS) on the DNA damage of human spermatozoa, the effects of 0,0. 0 6 2 5,0 .12 5,0 .2 5,0. 5 and 1.0 mM H 2 O 2 and 0,0 .1,1.0 , 2 .0,5. 0 mM of β NADPH in vitro treatment of sperm, single cell gel electrophoresis test detected exogenous H2 O2 and endogenous O · -2 caused sperm DNA strand breaks. The results showed that the percentage of comet cells increased significantly with the treatment of 1.0mMH 2 O 2 or 5.0mM NADPH for 30min to 4h, and the mean comet tails also increased significantly with obvious time-effect relationship. The percentage of comet cells in control group was significantly increased when sperm cells were treated with 0. 06 2 5 mM ~ 1.0 mM H 2 O 2 or 0.1 ~ 5.0 mM NDPH for 1 h. The average comet tails increased significantly with obvious dose-response relationship. These findings suggest that the exogenous H2 O2 and β NADPH stimulate the endogenous O · 2 produced by cells in vitro to cause sperm DNA strand breaks