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目的 :研究小牛类表皮生长因子活性肽 (C epidermalgrowthfactor likeactivepeptide,C EGF)在体内外对肝细胞再生的影响。方法 :从小牛颌下腺提取C EGF ,利用细胞培养及CCl4 诱导的大鼠肝坏死模型 ,检测了C EGF对肝细胞再生的刺激作用。结果 :C EGF可促进人肝癌细胞系SMMC 772 1细胞及小鼠胎肝细胞的增殖 ;在肝坏死动物模型中 ,C EGF可显著地启动并促进肝细胞的再生 ,C EGF对细胞增殖的刺激作用在体内外均可被胰岛素增强。结论 :C EGF在体内外对肝细胞的增殖均有促进作用 ,此结果为C EGF在肝病治疗中的应用提供了实验依据。
Objective: To study the effect of C epidermal growth factor similar activating peptide (C EGF) on hepatocyte regeneration in vitro and in vivo. Methods: C EGF was extracted from calf submandibular gland, and the stimulating effect of C EGF on hepatocyte regeneration was tested by cell culture and CCl4-induced hepatic necrosis model. Results: C EGF can promote the proliferation of human hepatocarcinoma cell line SMMC 772 1 cells and mouse fetal liver cells; in the animal model of hepatic necrosis, C EGF can be significantly activated and promote the regeneration of liver cells, stimulated by C EGF on cell proliferation The role of both in vivo and in vitro can be enhanced by insulin. CONCLUSION: C EGF can promote the proliferation of hepatocytes both in vitro and in vivo. This result provides an experimental basis for the application of C EGF in the treatment of liver diseases.