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目的:分析肺腺癌转移淋巴结与正常淋巴结组织蛋白质表达差异。方法:运用蛋白质组技术,对肺腺癌转移淋巴结与正常淋巴结组织蛋白质进行分离和比较分析,并在蛋白质和mRNA水平进行进一步验证。结果:获得了背景清晰、分辨率和重复性较好的双向凝胶电泳图谱,比较分析显示与正常淋巴结蛋白质表达水平相比,肺腺癌转移淋巴结有23个蛋白点的表达量存在明显差异,其中新增蛋白点4个,缺失1个,14个蛋白点表达水平明显上调,4个蛋白点表达水平明显下调。共鉴定了11个肺癌转移相关蛋白,其中,候选蛋白膜联蛋白1(ANX1)、细胞骨架蛋白(CK18)、Rho-GDP解离抑制剂1(GDIR)、原肌球蛋白3(TPMF)、蛋白谷氨酰胺γ-谷氨酰转移酶(TGLC)和白介素18(IL-18)在肺腺癌转移淋巴结显著低表达,而候选蛋白核氯离子通道蛋白1(CLI1)、蛋白质二硫键异构酶(ER60)、肌酸激酶、硫氧还蛋白过氧化物酶1(PDX1)和热休克蛋白60(CH60)在肺腺癌转移淋巴结表达水平比正常淋巴结明显提高。结论:肺腺癌转移淋巴结与正常淋巴结蛋白质表达存在明显差异,这11种蛋白质可能与肺腺癌淋巴结转移机制有关。
Objective: To analyze the difference of protein expression between metastatic lymph node and normal lymph node in lung adenocarcinoma. Methods: The proteins of lung adenocarcinoma metastatic lymph node and normal lymph node tissue were isolated and compared by proteome technology, and further verified by protein and mRNA levels. Results: Two-dimensional gel electrophoresis with clear background, good resolution and repeatability was obtained. Compared with the normal lymph node, the expression of 23 protein spots in lung adenocarcinoma metastatic lymph nodes was significantly different. Among them, 4 new protein spots, 1 deletion, 14 protein spots were significantly up-regulated, and 4 protein spots were significantly down-regulated. A total of 11 lung metastasis-associated proteins were identified, among which candidate proteins annexin 1 (ANX1), cytoskeletal protein (CK18), Rho-GDP dissociation inhibitor 1 (GDIR), tropomyosin 3 (TPMF) Protein glutamine γ-glutamyl transferase (TGLC) and interleukin 18 (IL-18) were significantly down-regulated in metastatic lymph nodes of lung adenocarcinoma, while the expression of candidate proteins nuclear chloride channel protein 1 (CLI1), protein disulfide bond The expression of ER60, creatine kinase, thioredoxin peroxidase 1 (PDX1) and heat shock protein 60 (CH60) in lung adenocarcinoma metastatic lymph nodes was significantly higher than that in normal lymph nodes. Conclusion: The expression of protein in metastatic lymph node and normal lymph node of lung adenocarcinoma is significantly different. These 11 proteins may be related to the mechanism of lymph node metastasis of lung adenocarcinoma.