A male-specific SCAR DNA marker and sex ratio of seedlings in salak(Salacca zalacca var.zalacca)

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A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. zalacca(salak palm). The marker is 1579 bp long and has a GC content of 38.5 %. Its sequence contains 1 or 2 open reading frames, indicating the marker is probably a coding region. No highly similar sequences were found in a search of the Gen Bank database.Sexes were identified using the SCAR DNA marker for three kinds of seedlings grouped by the number of seeds per fruit(1, 2 or 3). The sex ratio of female to male did not differ significantly from 1:1 for the three kinds of seedlings, implying that the number of seeds per fruit is not a reliable index to identify the sex of a seedling. A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. Zalacca (salak palm). The marker is 1579 bp long and has a GC content of 38.5%. Its sequence contains 1 or 2 open reading frames, indicating the marker is probably a coding region. No highly similar sequences were found in a search of the Gen Bank database. These were identified using the SCAR DNA marker for three kinds of seedlings grouped by the number of seeds per fruit ( 1, 2 or 3). The sex ratio of female to male did not differ significantly from 1: 1 for the three kinds of seedlings, implying that the number of seeds per fruit is not a reliable index to identify the sex of a seedling.
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