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目的探讨大蒜素(allicin)对人外周血内皮祖细胞(endothelial progenitor cells,EPCs)增殖活力及迁移能力的影响。方法提取健康成人EPCs,经DiI-acLDL和FITC-UEA-I染色后,于激光共聚焦显微镜下观察染色结果;向EPCs加入5、10、15和20μg/ml的大蒜素,分别作用12、24、48和72 h,MTT法检测各组EPCs的增殖活力;Transwell小室试验及Western blot法分别检测大蒜素作用48 h后,其对各组EPCs迁移能力及EPCs中血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质细胞衍生因子-1(stromal cells derived factor-1,SDF-1)蛋白表达水平的影响。结果 DiI-acLDL染色可见绿色荧光,FITC-UEA-I染色可见红色荧光,双重染色可见黄色荧光。作用12、24和48 h的10、15和20μg/ml大蒜素组EPCs的增殖活力显著高于空白对照组(P<0.05),且呈浓度依赖性,于48 h时达最高;10、15和20μg/ml大蒜素组EPCs的迁移能力及EPCs中VEGF和SDF-1蛋白的表达水平均明显高于空白对照组(P<0.05),且呈浓度依赖性。结论大蒜素可提高人EPCs的增殖活力及迁移能力,该作用可能是通过调节VEGF和SDF-1的表达而实现的,为动脉粥样硬化(atherosclerosis,AS)的防治提供了新的思路。
Objective To investigate the effect of allicin on proliferation and migration of human peripheral blood endothelial progenitor cells (EPCs). Methods EPCs were isolated from healthy adults and stained with DiI-acLDL and FITC-UEA-I. The staining results were observed under confocal laser scanning microscope. 5, 15, 15 and 20μg / ml allicin were added into EPCs, , 48 and 72 h respectively. The proliferation activity of EPCs in each group was detected by MTT assay. Transwell chamber assay and Western blotting were used to detect the migration of EPCs and the expression of vascular endothelial growth factor factor, VEGF and stromal cells derived factor-1 (SDF-1) Results DiI-acLDL staining showed green fluorescence, FITC-UEA-I staining showed red fluorescence, double staining visible yellow fluorescence. EPCs at 10, 15 and 20 μg / ml allicin treatment for 12, 24 and 48 h showed significantly higher proliferative activity than blank control group (P <0.05) in a concentration-dependent manner and reached the peak at 48 h. EPCs in 20μg / ml allicin group and VEGF and SDF-1 protein expression levels in EPCs were significantly higher than those in blank control group (P <0.05), and in a concentration-dependent manner. Conclusion Allicin can increase the viability and migration ability of human EPCs, which may be through the regulation of the expression of VEGF and SDF-1, and provide a new idea for the prevention and treatment of atherosclerosis (AS).