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目的:通过检测环孢素A(cyclosporin A,CsA)作用下牙龈组织TGF-β1和PCNA的表达情况,探讨CsA导致牙龈过度生长(gingival overgrowth,GO)可能的病理机制。方法:20只单侧拔除下颌磨牙的SD大鼠随机分为实验组(CsA,10mg.kg-1·d-1皮下注射)和对照组(等量生理盐水皮下注射)。4周后处死动物,截取下颌磨牙区组织用以检测。体视显微镜下测量牙龈宽度;标本脱钙后制片,经HE染色,行牙龈组织学镜检测量;免疫组化方法(ABC法)检测标本牙龈TGF-β1、PCNA的表达情况。以Image Pro-plus 6.0图像分析软件,对免疫组化检测结果作半定量分析,并以SAS 6.12软件包进行统计学处理。结果:实验组用药后4周,有牙侧与无牙侧牙龈TGF-β1、PCNA蛋白表达水平升高,与对照组比较具有显著差异;各组内有牙侧与无牙侧牙龈的TGF-β1、PCNA表达水平无显著差异。结论:CsA导致GO的过程中,牙龈TGF-β1表达水平上调及其作用可能独立于牙龈炎症,CsA有刺激牙龈成纤维细胞过量表达TGF-β1的作用;CsA促进牙龈上皮增殖活性的提高,可能是上皮细胞数量增加、上皮层增厚的主要原因。
OBJECTIVE: To investigate the possible pathological mechanism of CsA-induced gingival overgrowth (GO) by detecting the expression of TGF-β1 and PCNA in gingival tissues under the action of cyclosporin A (CsA). Methods: Twenty SD SD rats with unilateral mandibular molar removal were randomly divided into experimental group (CsA, 10 mg.kg-1 · d-1 subcutaneously) and control group (NS injected subcutaneously). After 4 weeks, the animals were sacrificed and the mandibular molars were intercepted for testing. Gingival width was measured under stereomicroscope. Specimens were prepared by decalcification and examined by gingival histology with HE staining. The expression of TGF-β1 and PCNA in gingival tissues was detected by immunohistochemistry (ABC method). The results of immunohistochemistry were semi-quantitatively analyzed with Image Pro-plus 6.0 image analysis software and statistically analyzed with SAS 6.12 software package. Results: After 4 weeks of treatment, the expressions of TGF-β1 and PCNA in the gingival tissues of the experimental group and the non-gingival tissues were significantly higher than those of the control group. In the experimental group, the expressions of TGF- β1, PCNA expression levels were not significantly different. CONCLUSIONS: The up-regulation of gingival TGF-β1 expression and its effect may be independent of gingival inflammation during CsA-induced GO. Gingival fibroblast can up-regulate the expression of TGF-β1 by CsA. CsA may promote the proliferation of gingival epithelial cells. Is the number of epithelial cells, the main reason for the thickening of the epithelium.