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目的 :研究血管紧张素Ⅱ (AngⅡ )预处理是否对缺血 /再灌注 (I/R)心肌有保护作用 ,其作用是否通过蛋白激酶C(proteinkinaseC ,PKC)及线粒体ATP依赖性钾通道 (KATP)而起作用。方法 :4 0只SD大鼠 ,随机分为 5组 (每组各 8只 ) :AngⅡ预处理组 (APC组 )、缬沙坦加AngⅡ预处理组 (VAPC组 )、PKC阻滞剂chelerythine加AngⅡ预处理组 (CLT组 )、线粒体KATP通道阻滞剂 5 Hydroxydecanoate加AngⅡ预处理组 (5 HD组 )和对照组(C组 )。应用Langendorff主动脉逆行灌流的体外大鼠I/R心脏模型 ,观察各组大鼠I/R后心肌细胞乳酸脱氢酶(LDH)及肌酸激酶 (CK)漏出率、心肌组织三磷酸腺苷 (ATP)含量及心功能指标 (LVSP与±dp/dtmax)。结果 :APC组CK、LDH漏出率较C组明显减少 (P <0 .0 1) ,心肌ATP含量较C组增加 (P <0 .0 1) ,心功能 (LVSP与±dp/dtmax)较C组改善。VAPC组、CLT组及 5 HD组上述各指标 (LDH、CK、ATP、LVSP及±dp/dtmax)分别与C组相应的各指标比较差异无统计学意义 (P >0 .0 5 )。结论 :AngⅡ预处理对I/R心肌有保护作用
OBJECTIVE: To investigate whether AngⅡ preconditioning has a protective effect on myocardial ischemia / reperfusion (I / R) and whether its effect is mediated by proteinkinase C (PKC) and mitochondrial ATP-dependent potassium channel (KATP) ) Works. Methods: 40 SD rats were randomly divided into 5 groups (8 in each group): AngⅡ pretreatment group (APC group), Valsartan plus Ang Ⅱ pretreatment group (VAPC group), PKC blocker chelerythine plus Ang Ⅱ pretreatment group (CLT group), mitochondrial KATP channel blocker 5 Hydroxydecanoate plus Ang Ⅱ pretreatment group (5 HD group) and control group (C group). The Langendorff aortic retrograde perfusion in vitro rat I / R cardiac model was used to observe the changes of lactate dehydrogenase (LDH) and creatine kinase (CK) leakage rate, myocardial ATPase (ATP) Content and cardiac function (LVSP and ± dp / dtmax). Results: The leakage rate of CK and LDH in APC group was significantly lower than that in C group (P <0.01), and the content of ATP in myocardial tissue was higher than that in C group (P <0.01). The LVSP and ± dp / dtmax Group C improved. The above indexes (LDH, CK, ATP, LVSP and ± dp / dtmax) of VAPC group, CLT group and 5 HD group had no significant difference with those of C group (P> 0.05). Conclusion: Ang Ⅱ preconditioning has a protective effect on I / R myocardium