[Objective] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron(C.A.Mey.).[Method] H.ammodendron(C.A.Mey.) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings.[Result] Sterilization of H.ammodendron(C.A.Mey.) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min.Seeds with diameter >2.0 mm were used as explants and achieved relatively high survival rate of aseptic seedlings,which reached above 65%.[Conclusion] This study established a surface sterilization method for tissue culture of H.ammodendron(C.A.Mey.) Bunge seeds. [Objective] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron (CAMey.). [Method] H.ammodendron (CAMey.) Seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [Result] Sterilization of H. ammodendron (CAMey.) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min .Seeds with diameter > 2.0 mm were used as explants and achieved relatively high survival rate of aseptic seedlings, which reached above 65%. [Conclusion] This study established a surface sterilization method for tissue culture of H.ammodendron (CAMey.) Bunge seeds.