论文部分内容阅读
目的了解EB病毒相关性胃癌(EBVaGC)与非EB病毒相关性胃癌(EBVnGC)在基因表达上的差异,探讨EB病毒可能的致胃癌机制。方法对155例连续胃腺癌患者癌组织用原位杂交(ISH)技术检测EB病毒小RNA(EBER)的表达,确定其中的EBVaGC。用免疫组化染色检测所有组织切片中P53、P21WAF1蛋白的表达。结果10例(6·45%)胃腺癌EBERISH结果阳性,属EBVaGC;其中4例P53表达呈微弱阳性,显著低于EBVnGC组[58例(40%)P53显著阳性,30例(21%)P53微弱阳性];7例(7/10)EBVaGC癌组织中P21WAF1蛋白染色阳性,显著高于EBVnGC组P21WAF1蛋白阳性表达率[55例(38%)P21WAF1蛋白染色阳性]。结论EBVaGC有独特的癌变分子机制,P53基因突变不起主要作用。
Objective To investigate the differences in gene expression between Epstein-Barr virus-associated gastric cancer (EBVaGC) and non-Epstein-Barr virus (EBVnGC) related gastric cancer (EBVnGC) and to explore the possible mechanism of EB virus-induced gastric cancer. Methods The expression of Epstein-Barr virus RNA (EBER) was detected by in situ hybridization (ISH) in 155 cases of patients with continuous gastric adenocarcinoma and the EBVaGC was determined. Immunohistochemical staining was used to detect the expression of P53 and P21WAF1 protein in all tissue sections. Results The positive results of EBERISH in 10 cases (6.45%) of gastric adenocarcinoma were EBVaGC. The expression of P53 was weakly positive in 4 cases (P <0.05), significantly lower than that in EBVnGC group (58 cases, 40% Weakly positive]. P21WAF1 protein was positive in 7 (7/10) EBVaGC cancer tissues, which was significantly higher than that in EBVnGC group [55 (38%) P21WAF1 positive). Conclusion EBVaGC has a unique molecular mechanism of carcinogenesis, P53 gene mutation does not play a major role.