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本文主要研究水稻稻瘟病菌(PyriculariaOryzae)粗毒素提取液对水稻愈伤组织分化的抑制效应。愈伤组织的每个细胞都可当作诱发突变体的独立单位。可在含粗毒素的分化培养基上,诱发抗性细胞突变。从而可由花粉愈伤组织中选出抗稻瘟病的突变体。材料和方法材料:选用上海地区感病品种桂农12和花寒早作为花药培养的起始材料,然后将其愈伤组织作为本试验的材料。取同一天诱导、大小一致的愈伤组织作为受体,在试管内加入粗毒素提取液0.2ml/管。方法:E_3和F_1粗毒素提取液的置备见前文(郑祖玲等1984)。A、B粗毒素提取液分二种处理:1.母液;2.稀释至1/10。对照为20％土豆液和培养液(Tamari和Kaji,1954)两种。
In this paper, we studied the inhibitory effect of crude extracts of Pyricularia oryzae on rice callus differentiation. Each cell of the callus can be used as an independent unit to induce the mutant. Resistant cell mutations can be induced on crude toxin-containing differentiation media. Thus, the blast resistant mutant can be selected from pollen callus. MATERIALS AND METHODS MATERIALS: The susceptible varieties Guinong 12 and Huananzao in Shanghai were selected as the starting material for anther culture, and then the callus was used as the material of this experiment. Take the same day induction, the same size of callus as a receptor, in the test tube was added crude extract 0.2ml / tube. Methods: The preparation of E_3 and F_1 crude toxin extracts was previously described (Zheng, Zuoling et al., 1984). A, B crude toxin extract in two ways: 1. mother liquor; 2. diluted to 1/10. The control was 20% potatoes and broth (Tamari and Kaji, 1954) two kinds.