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目的了解2009年豫北地区手足口病(HFMD)流行的病原。方法采集HFMD患儿的疱疹液、粪便、咽拭子标本进行病毒培养分离,阳性毒株用肠道通用引物和肠道病毒71型(EV71)、柯萨奇病毒A组16型(CA16)特异性引物进行反转录-PCR鉴定。结果 1～5岁儿童是肠道病毒感染的高危人群,占所有感染者的89.53%(214/239例)。EV71感染占所有感染EV的63.59%(152/239例),CA16占所有EV感染病例的25.94%(62/239例)。不同性别儿童EV阳性率和EV71阳性率无统计学差异。利用恶性胚胎横纹肌瘤细胞和人喉癌细胞株共分离到阳性毒株315株,总阳性率为60.46%(315/521例)。其中恶性胚胎横纹肌瘤细胞299株,阳性率为57.3.9%(297/521例);人喉癌细胞株16株,阳性率为3.07%(16/521例)。152例分离到EV71病毒,感染率为39.48%;62例分离到CA16,感染率为16.10%。结论引起豫北地区2009年HFMD主要病原是EV71和CA16。
Objective To understand the prevalence of hand-foot-mouth disease (HFMD) in North Henan in 2009. Methods The herpes fluid, feces and throat swab samples of children with HFMD were collected for virus culture. The positive strains were detected by using universal primers and enterovirus 71 (EV71) and Coxsackievirus A group 16 (CA16) Sex primers were reverse transcriptase-PCR identified. Results Children aged 1-5 years were at high risk of enterovirus infection, accounting for 89.53% (214/239 cases) of all infected persons. EV71 infection accounted for 63.59% (152/239 cases) of all infected EVs, and CA16 accounted for 25.94% (62/239 cases) of all EV infections. There was no significant difference in EV positive rate and EV71 positive rate among children of different genders. A total of 315 positive strains were isolated from malignant embryonic rhabdomyoma and human laryngeal carcinoma cell lines, with a total positive rate of 60.46% (315/521 cases). Among them, 299 were malignant embryonic rhabdomyoma, with a positive rate of 57.3.9% (297/521 cases) and 16 human laryngeal carcinoma cell lines with a positive rate of 3.07% (16/521 cases). 152 cases isolated EV71 virus, the infection rate was 39.48%; 62 cases were isolated CA16, the infection rate was 16.10%. Conclusion The major pathogens causing HFMD in northern Henan in 2009 are EV71 and CA16.