论文部分内容阅读
本文建立了微波提取细梗胡枝子不同药用部位中黄酮类化合物,并以分光光度法测定其含量的实验方法。在微波单因素提取的基础上,采取L9(34)正交设计优化实验,优化了萃取乙醇浓度、液固比、微波功率和微波辐射时间等提取条件,确定了微波辅助萃取法提取细梗胡枝子样品中叶、枝和根等不同药用部位中的黄酮类化合物的最佳工艺参数分别为:叶:微波功率40%(340W),乙醇浓度为50%,液固比为80 ml/g,提取时间6 min;枝:微波功率40%(340W),乙醇浓度为50%,液固比为80 ml/g,提取时间5 min;根:微波功率50%(425W),乙醇浓度为60%,液固比为90 ml/g,提取时间5 min。在最优条件下测得黄酮类化合物含量分别为:10.43%(叶),5.91%(枝),(根)5.29%。在上述最优条件下进行了重复性和加样回收率实验,与索氏提取法、超声波提取法以及乙醇冷浸法比较,微波辅助萃取法具有操作简便、快速、重现性好等特点。
In this paper, an experimental method for the determination of flavonoids in different medicinal parts of Lespedeza brassicae by microwave was established and its content was determined by spectrophotometry. On the basis of microwave single factor extraction, L9 (34) orthogonal design optimization experiments were adopted to optimize extraction conditions such as ethanol concentration, liquid-solid ratio, microwave power, and microwave irradiation time, and microwave-assisted extraction was used to extract fine stem hulls. The optimum technological parameters of flavonoids in leaves, branches and roots of different branches in the sample were: leaf: microwave power 40% (340W), ethanol concentration 50%, liquid to solid ratio 80 ml/g, Extraction time 6 min; branch: microwave power 40% (340W), ethanol concentration 50%, liquid-solid ratio 80 ml/g, extraction time 5 min; root: microwave power 50% (425W), ethanol concentration 60% The liquid-solid ratio is 90 ml/g and the extraction time is 5 min. The contents of flavonoids measured under optimal conditions were: 10.43% (leaf), 5.91% (branch), and (root) 5.29%. The reproducibility and sample recovery experiments were carried out under the above optimal conditions. Compared with Soxhlet extraction, ultrasonic extraction and ethanol cold leaching, the microwave-assisted extraction method was simple, rapid, and reproducible.