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目的探讨天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、骨桥蛋白(OPN)和埃兹蛋白(Ezrin)在乳腺浸润性导管癌(IDC)中的表达及其与临床病理因素的关系。方法应用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法检测71例术中切除的原发性乳腺IDC和33例距癌灶约5 cm处正常乳腺组织中Caspase-3、OPN和Ezrin的表达,分析3种蛋白间及其与乳腺IDC病理特征的关系。结果乳腺IDC组Caspase-3、OPN及Ezrin的阳性表达率分别为43.66%、74.65%、64.79%,正常乳腺组织对照组Caspase-3、OPN及Ezrin阳性表达率分别为75.76%、33.33%、39.39%,3指标在两组间表达差异均有统计学意义(P<0.05);在IDC组,3种蛋白的表达与乳腺IDC的淋巴结转移及肿瘤复发相关;Caspase-3、OPN及Ezrin蛋白的表达与雌激素受体(ER)、孕激素受体(PR)及原癌基因人类表皮生长因子受体2(Her-2)的表达无明显相关性;Caspase-3与OPN、Ezrin的表达呈负相关(r=-0.258、-0.313),OPN与Ezrin的表达呈正相关(r=0.29)。结论乳腺浸润性导管癌中Caspase-3、OPN、Ezrin 3种蛋白的异常表达在其发生、发展中可能起着重要的作用。
Objective To investigate the expression of caspase-3, osteopontin (OPN) and Ezrin in invasive ductal carcinoma of the breast (IDC) The relationship between pathological factors. Methods Immunohistochemical streptavidin-peroxidase (SP) method was used to detect the expression of Caspase-3 in 71 cases of primary breast carcinoma (IDC) and 33 cases of normal breast tissue , OPN and Ezrin, and to analyze the relationship between the three proteins and the pathological features of breast IDC. Results The positive rates of Caspase-3, OPN and Ezrin in breast IDC group were 43.66%, 74.65% and 64.79%, respectively. The positive rates of Caspase-3, OPN and Ezrin in normal breast tissue were 75.76%, 33.33% and 39.39 (P <0.05). In IDC group, the expressions of three proteins were correlated with lymph node metastasis and tumor recurrence in breast IDC. The expression of Caspase-3, OPN and Ezrin protein There was no significant correlation between the expression of ER-1, PR and PR-2 (Her-2). The expressions of Caspase-3, OPN and Ezrin Negative correlation (r = -0.258, -0.313), the expression of OPN and Ezrin was positively correlated (r = 0.29). Conclusion Abnormal expression of three proteins Caspase-3, OPN and Ezrin in invasive ductal carcinoma of the breast may play an important role in their occurrence and development.