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茎瘤芥(榨菜)具有瘤茎膨大的性状,该特征是一种重要的经济性状。在前期研究中,我们获得一个可能与瘤茎膨大有关的基因片段,该基因与拟南芥PKS1基因相似。通过RACE技术获得了该基因全长序列,命名为BjPKS1。该基因的mRNA序列全长1440bp,其预测的ORF长度为1131bp,编码的氨基酸序列长度为376aa。多序列比对发现,预测的氨基酸序列与其他物种的PKS基因家族蛋白具有高度相似性,在多个区域都高度保守,进化分析显示Bj PKS与拟南芥的PKS1基因关系最近。荧光定量RT-PCR结果显示,该基因在茎瘤芥瘤茎膨大发育的过程中表达量明显上升。将该基因转化拟南芥,转基因植株株高和节间都明显缩短。结果表明BjPKS1可能具有其他物种中同源基因相似的分子功能,其在茎瘤芥瘤茎膨大过程中的作用有待进一步研究。
Tuber mustard (tuber mustard) with tuberculous features, this feature is an important economic traits. In the previous study, we obtained a gene fragment possibly related to tumor stem enlargement, which is similar to the Arabidopsis PKS1 gene. The full-length sequence of this gene was obtained by RACE technology and named BjPKS1. The mRNA sequence of this gene is 1440bp in length, with a predicted ORF length of 1131bp and an encoded amino acid sequence of 376aa. Multiple sequence alignment revealed that the predicted amino acid sequence is highly similar to PKS gene family proteins of other species and highly conserved in multiple regions. Phylogenetic analysis showed that Bj PKS has the closest relationship with PKS1 gene in Arabidopsis. Fluorescent quantitative RT-PCR results show that the gene expression in the tumor stem tumor enlargement and development significantly increased. When the gene was transformed into Arabidopsis thaliana, the plant height and internode of transgenic plants were significantly shortened. The results showed that BjPKS1 may have similar molecular functions of homologous genes in other species, and its role in tumor stem tumor enlargement needs further study.